Vantage offers Taqman genotyping for single SNPs or short indels as well as copy number variant detection for certain genes. We run pre-designed assays or design custom assays depending on the target sequence for most SNPs. Customers should submit their list of target SNPs as rsID’s through iLab. Data will be reported as an excel file containing the reported genotyping results for each SNP as well as the context sequence and uploaded to the iLab project when complete.
Vantage offers various options for DNA extraction from blood and saliva samples that can then be processed for genotyping. Please inquire further about these options. The investigator can also have previously extracted DNA from outside of VANTAGE brought or shipped to the core for genotyping. As a rule of thumb, your DNA should have a concentration of 20ng/ul by Nanodrop or pico green quantitation and have a minimum volume of 25ul, however, DNA amounts and plating requirements will vary based on your genotyping needs.
Please contact Karen Beeri at karen.beeri@vumc.org for consultation.