Scientific Director: James Crowe, Jr, M.D.
Managing Director: David Flaherty
Core Aims:
- Provide access to state-of-the art equipment for analysis of cell phenotype and properties
- Provide access to state-of-the art equipment for sorting cells or sub-cellular elements
- Furnish professional guidance in experimental design and data interpretation
- Assist users in sample preparation of cells, sub-cellular preparations, and secreted factors for optimal analysis
- Trains users, on a continuing basis, in development of protocols and basic and advanced use of the flow cytometry equipment and software
- Assist users with grant and publication material, data analysis, and figure preparation
- Provide digestive disease specific training, troubleshooting, support and optimization to VDDRC members via the Flow Cytometry Core
Core Services:
• Training, acquisition and analysis on our state-of-the-art cytometers for experiments that include up to 17 different fluorochromes simultaneously
• High-speed sorting with up to 15 different fluorochromes simultaneously with the ability to sort and or clone into any plate or dish
• Mass Cytometry with the potential to measure 30-100 antigens simultaneously
•Three levels of biocontainment to address the needs of every DDRC member
• Imaging Cytometry
• Expert consultation and troubleshooting and experimental design guidance
For additional information on the Vanderbilt Flow Cytometry and Immunology Shared resource please visit www.vmcflow.com
