LABORATORY PROTOCOLS

IT'S HERE NOW! ...   Updated Mouse Natural Killer T Cell Protocols    http://onlinelibrary.wiley.com/doi/10.1002/cpim.22/pdf

Kumar, A., Bezbradica, J.S., A.K. Stanic and S. JOYCE (2017) Chapter 14-Unit 14.13: Isolation and functional use of mouse semi-invariant natural killer T cells. Current Protocols Immunology 14.13.1---14.13.55

 

Mouse Natural Killer T Cell Protocols     2006 JSBezbradica et al CPI.pdf

Bezbradica, J.S., A.K. Stanic and S. JOYCE (2006) Chapter 14-Unit 14.13: Isolation and functional use of mouse NK T cells. Current Protocols Immunology 14.13.1---14.13.27 

http://onlinelibrary.wiley.com/doi/10.1002/0471142735.im1413s73/full

 

p/MHC class I Tetramer Protocols

Rodenko, B., M. Toebes, S.R. Hadrup, W.J. van Esch, A.M. Molenaar, T.N. Schumacher and H. Ovaa (2006) Generation of peptide-­MHC class I complexes through UV-­mediated ligand exchange. Nature Protocols 1: 1120-­‑1132

http://www.nature.com/nprot/journal/v1/n3/full/nprot.2006.121.html

 

Eliciting and Characterizing Lung-Resident CD8+ T cells    2016_PGilchuk_mmb_Protocols.pdf

Gilchuk, P., F.C. Knight, J.T. Wilson and S. JOYCE (2016) Eliciting epitope-specific CD8+ T cell response by immunization with microbial protein antigens formulated with alpha-galactosylceramide: theory, practice, & protocols. In C.B. Fox ed., Methods in Molecular Biology: Vaccine Adjuvant — Methods and Protocols. Springer Science & Business Media, LLC, NY, New York; vol 1494, pages 321--352

http://link.springer.com/protocol/10.1007/978-1-4939-6445-1_25/fulltext…